화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.25, No.5, 1088-1093, September, 2008
DOI10.1007/s11814-008-0178-z  Export Citation
Xylitol fermentation by Candida subtropicalis WF79 immobilized in polyacrylic hydrogel films
Wen-Chang Liaw, Wen-Shion Chang1, Kuan-Pin Chen, Yee-Fan Chen2, and Chee-Shan Chen2, †
  • Department of Chemical Engineering, National Yunlin University of Science and Technology, 123, Section 3, University Road, Touliu, Yunlin 640, Taiwan, R.O.C.
  • 1Jin Wen Institute of Technology, 99, An-Chung Road, Shin-Tien, Taipei County, 231, Taiwan, R.O.C.
  • 2Department of Applied Chemistry, Chaoyang University of Technology, 168, Gifeng E. Rd., Wufeng, Taichung County, 413, Taiwan, R.O.C.
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Polyacrylate hydrogel films were used as immobilization matrices for cell entrapment of the yeast strain, Candida subtropicalis WF79, isolated from sugarcane bagasse. The hydrogel films with immobilized viable yeast cells were employed for the bioconversion from xylose to xylitol. The hydrogel was prepared from the monomers of 2-hydroxyethyl methacrylate (HEMA), methacrylic acid (MAA), and N,N-dimethyl acrylamide (DMA) with polyethylene glycol diacrylate (PEG-DA, weight-average molecular weight Mw=400 and 1,000 g/mol) as the crosslinking agent. The mechanical properties and fermentation yields of the immobilized polyacrylate hydrogel film with different monomeric formulation were investigated. Fermentation was carried out in a medium of 100 mL aqueous solution containing various amount of xylose. The conversion rate of xylose to xylitol conversion reached a maximum value of 80% after 120 h. The bioconversion activity gradually declined, after 720 h, to 65% conversion at 1,080 h.
Keywords:Xylitol;Polyacrylate;Hydrogel;Immobilization;Candida
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