Inhibition of melanogenesis by Erigeron canadensis via down-regulating melanogenic enzymes in B16F10 melanoma cells

Eun-Suk Hong; Duc Thi Minh Nguyen; Dung Hoang Nguyen; Eun-Ki Kim

Korean Journal of Chemical Engineering, Vol.25, No.6, 1463-1466, November, 2008

DOI10.1007/s11814-008-0240-x Export Citation

Inhibition of melanogenesis by Erigeron canadensis via down-regulating melanogenic enzymes in B16F10 melanoma cells

Eun-Suk Hong, Duc Thi Minh Nguyen, Dung Hoang Nguyen, and Eun-Ki Kim^†

Department of Biological Engineering, Inha University, Incheon 402-751, Korea

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The effects of Erigeron canadensis extract on melanogenesis and cell toxicity in cultured B16F10 mouse melanoma cells were investigated. E. canadensis extract down regulated melanin synthesis effectively at a non-toxic concentration. Its extract was fractionated by using a recycling HPLC with GS310 column (21.5×500 mm, 10-15 μM) into five fractions. The fraction 1 showed melanin inhibition by 48.0% at 100 mg/ml which was 2.5 times more efficient than the depigmenting effect of commercial arbutin (17.5%) and also did not show cell toxicity. To elucidate the depigmenting mechanism of fraction 1, in vitro and cellular tyrosinase activity, antioxidant activity, and protein level of the main melanogenic enzymes, such as tyrosinase, TRP-1 and TRP-2 were evaluated. Fraction 1 inhibited melanin synthesis in B16F10 melanoma cells by decreasing protein levels of melanogenic enzymes, especially tyrosinase. In conclusion, we suggest that this fraction may be a safe and effective depigmentation agent.

Keywords:Erigeron canadensis;Melanogenesis;Depigmenting;Tyrosinase

[References]

Bell AA, Weeler MH, Ann. Rev. Phytophathol., 24, 411 (1986)
Lerner AB, Fitzpatrick TB, Physiol. Rev., 30, 91 (1950)
Prota G, Melanins and melanogenesis, Academic Press, New York (1992)
Lopez JNR, Tudela J, Varon R, Carmona FG, Canovas FG, J. Biol. Chem., 267, 3901 (1992)
Imokawa G, Mishima Y, Cancer Res., 42, 1994 (1982)
Masuda M, Tejima T, Suzuki T, Cosmetics and Toiletries., 111, 65 (1996)
Briganti S, Camera E, Picardo M, Pigment Cell Res., 16, 101 (2003)
Parvez S, Kang MK, Chung HS, Cho CW, Hong MC, Shin MK, Bae HS, Phytother. Res., 20, 921 (2006)
Curto EV, Kwong C, Hermersdorfer H, Glatt H, Santis C, Virador V, Hearing VJ, Dooley TP, Biochem. Pharmacol., 15 (1999)
Nakagawa M, Kawai K, Contact Dermatitis., 32, 9 (1995)
Ortonne JP, Camacho F, Wainwright N, Bergfelt L, Westerhof W, Roseeuw D, Cutis, 74, 67 (2004)
Lee SH, Choi SY, Kim HC, Hwang JS, Biol. Pharm. Bull., 25, 1045 (2002)
Roh JS, Han JY, Kim JH, Hwang JK, Biol. Pharm. Bull., 27, 1976 (2004)
Khan SB, Haq AU, Afza N, Malik A, Khan MTH, Shah MR, Choudhary MI, Chem. pharm. Bull., 53, 86 (2005)
Masamoto Y, Murata Y, Baba K, Shimoishi Y, Tada M, Takahata K, Biol. Pharm. Bull., 27, 422 (2004)
Itoh T, Furuichi Y, Biosci. Biotechnol. Biochem., 69, 873 (2005)
Blois MS, Nature, 1981, 1199 (1958)
Chun HJ, Hwang SG, Jeong DH, Baek SH, Jeon BH, Woo WH, J. Pharm. Soc. Kor., 46, 137 (2002)
Martinez-esparza M, Jimenez-Cervantes C, Solano F, Lozano JA, Garcia-Borron JC, Eur. J. Biochem., 255, 139 (1998)

[Referenced By]

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[1] Bell AA, Weeler MH, Ann. Rev. Phytophathol., 24, 411 (1986)

[2] Lerner AB, Fitzpatrick TB, Physiol. Rev., 30, 91 (1950)

[3] Prota G, Melanins and melanogenesis, Academic Press, New York (1992)

[4] Lopez JNR, Tudela J, Varon R, Carmona FG, Canovas FG, J. Biol. Chem., 267, 3901 (1992)

[5] Imokawa G, Mishima Y, Cancer Res., 42, 1994 (1982)

[6] Masuda M, Tejima T, Suzuki T, Cosmetics and Toiletries., 111, 65 (1996)

[7] Briganti S, Camera E, Picardo M, Pigment Cell Res., 16, 101 (2003)

[8] Parvez S, Kang MK, Chung HS, Cho CW, Hong MC, Shin MK, Bae HS, Phytother. Res., 20, 921 (2006)

[9] Curto EV, Kwong C, Hermersdorfer H, Glatt H, Santis C, Virador V, Hearing VJ, Dooley TP, Biochem. Pharmacol., 15 (1999)

[10] Nakagawa M, Kawai K, Contact Dermatitis., 32, 9 (1995)

[11] Ortonne JP, Camacho F, Wainwright N, Bergfelt L, Westerhof W, Roseeuw D, Cutis, 74, 67 (2004)

[12] Lee SH, Choi SY, Kim HC, Hwang JS, Biol. Pharm. Bull., 25, 1045 (2002)

[13] Roh JS, Han JY, Kim JH, Hwang JK, Biol. Pharm. Bull., 27, 1976 (2004)

[14] Khan SB, Haq AU, Afza N, Malik A, Khan MTH, Shah MR, Choudhary MI, Chem. pharm. Bull., 53, 86 (2005)

[15] Masamoto Y, Murata Y, Baba K, Shimoishi Y, Tada M, Takahata K, Biol. Pharm. Bull., 27, 422 (2004)

[16] Itoh T, Furuichi Y, Biosci. Biotechnol. Biochem., 69, 873 (2005)

[17] Blois MS, Nature, 1981, 1199 (1958)

[18] Chun HJ, Hwang SG, Jeong DH, Baek SH, Jeon BH, Woo WH, J. Pharm. Soc. Kor., 46, 137 (2002)

[19] Martinez-esparza M, Jimenez-Cervantes C, Solano F, Lozano JA, Garcia-Borron JC, Eur. J. Biochem., 255, 139 (1998)