Gene expression profiling in streptozotocin-induced diabetic rat liver in response to fungal polysaccharide treatment

Hye Jin Hwang; Sang Woo Kim; Yu Mi Baek; Sung Hak Lee; Hee Sun Hwang; Jong Won Yun

Korean Journal of Chemical Engineering, Vol.26, No.1, 115-126, January, 2009

DOI10.1007/s11814-009-0018-9 Export Citation

Gene expression profiling in streptozotocin-induced diabetic rat liver in response to fungal polysaccharide treatment

Hye Jin Hwang, Sang Woo Kim, Yu Mi Baek, Sung Hak Lee, Hee Sun Hwang, and Jong Won Yun^†

Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk 712-714, Korea

E-mail:

We established the gene expression profiling in streptozotocin (STZ)-induced diabetic rat liver in response to hypoglycemic fungal polysaccharides (EPS), using oligonucleotide microarray analysis. Differentially regulated genes showing higher fold change than 2 were identified and categorized through hierarchical clustering analysis. Among the 835 genes analyzed, 244 were up-regulated, while 321 were down-regulated after diabetes induction. Interestingly, many gene expressions altered after STZ-treatment mimicked a normal rat liver by EPS therapy. Most of these genes included genes involving cell structure and motility, immunity and defense, lipid, fatty acid and steroid metabolism, protein metabolism and modification, and signal transduction. More importantly, we found a total of 36 genes that showed significant fold changes in their expression that have not previously been examined in the context of diabetes. To validate the microarray results, we further confirmed the gene expression patterns by RT-PCR using four genes of interest (carboxylesterase 2, stearoyl-coenzyme A desaturase 1, insulin-like growth factor 1, and insulin-like growth factor binding protein 2). Taken together, EPS acted as a potent regulator of gene expression for a wide variety of genes in diabetic rat liver.

Keywords:Diabetes Mellitus;Fungal Polysaccharides;Gene Profile;Microarray;Streptozotocin

[References]

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Ohtsuka Y, Agishi Y, Nippon Rinsho., 50, 1631 (1992)
El-Demerdash FM, Yousef MI, El-Naga NI, Toxicol., 43, 57 (2005)
Potter PM, Wolverton JS, Morton CL, Whipple DO, Danks MK, Cytometry, 32, 223 (1998)
Zhu W, Song L, Zhang H, Matoney L, Lecluyse E, Yan B, Drug Metab. Dispos., 28, 186 (2000)
Yamada T, Hosokawa M, Sotoh T, Moroo I, Takahashi M, Akatsu H, Brain Res., 658, 163 (1994)
Satoh T, Hosokawa M, Annu. Rev. Pharmacol. Toxicol., 38, 257 (1998)
Xu G, Zhang Q, Ma MK, McLeod HL, Clin. Cancer Res., 8, 2605 (2002)
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Foster DW, Ann. NY Acad. Sci., 1033, 1 (2004)
Nishida M, Sasaki T, Terada H, Kawada J, Experientia., 44, 756 (1988)
Waters KM, Ntambi JM, J. Biol. Chem., 269, 27773 (1994)
Ramesh B, Pugalendi KV, Yale J. Biol. Med., 78, 189 (2005)
Ooi GT, Tseng LY, Rechler MM, Biochem. Biophys. Res. Commun., 189, 1031 (1992)
Rodgers BD, Bautista RM, Nicoll CS, Proc. Soc. Exp. Biol. Med., 210, 234 (1995)

[1] Shmueli E, Record CO, Alberti KG, Baillieres. Clin. Endocrinol. Metab., 6, 719 (1992)

[2] Chatila R, West AB, Medicine (Baltimore), 75, 327 (1996)

[3] Feingold KR, Wiley MH, MacRae G, Moser AH, Lear SR, Siperstein MD, Diabetes, 31, 388 (1982)

[4] McLennan SV, Heffernan S, Wright L, Rae C, Fisher E, Yue DK, Turtle JR, Diabetes, 40, 344 (1991)

[5] Saxena AK, Srivastava P, Kale RK, Baquer NZ, Biochem. Pharmacol., 45, 539 (1993)

[6] Hwang HJ, Kim SW, Baek YM, Lee SH, Hwang HS, Kumar SG, Rahman MA, Yun JW, Korean J. Chem. Eng., 25(2), 308 (2008)

[7] Yamamoto Y, Nakajima M, Yamazaki H, Yokoi T, Life Sci., 70, 471 (2001)

[8] Tolman KG, Chandramouli J, Clin Liver Dis., 7, 369 (2003)

[9] Attele AS, Zhou YP, Xie JT, Wu JA, Zhang L, Dey L, Pugh W, Rue PA, Polonsky KS, Yuan CS, Diabetes, 51, 1851 (2002)

[10] Xie JT, Aung HH, Wu JA, Attele AS, Yuan CS, Am. J. Chin. Med., 30, 187 (2002)

[11] Xie JT, Zhou YP, Dey L, Attele AS, Wu JA, Gu M, Phytomedicine, 9, 254 (2002)

[12] Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK, Curr. Med. Chem., 13, 1203 (2006)

[13] Hwang HJ, Kim SW, Lim JM, Joo JH, Kim HO, Kim HM, Yun JW, Life Sci., 76, 3069 (2005)

[14] Kim SW, Hwang HJ, Cho EJ, Oh JY, Baek YM, Choi JW, Yun JW, J. Proteome Res., 5, 2966 (2006)

[15] Kim SW, Hwang HJ, Kim HM, Lee MC, Lee MS, Choi JW, Yun JW, Proteomics, 6, 5291 (2006)

[16] Fathman CG, Soares L, Chan SM, Utz PJ, Nature, 435, 605 (2005)

[17] Gomase VS, Tagore S, Kale KV, Curr Drug Metab., 9, 221 (2008)

[18] Garcia-Escudero R, Paramio JM, Mol. Carcinog., 6, Epub ahead of Print (2008)

[19] Jakupciak JP, Dakubo GD, Maragh S, Parr RL, Curr. Opin. Mol. Ther., 8, 500 (2006)

[20] Iida S, Sato Y, Nakaya A, Shinohara Y, Hayashi Y, Sawada A, Nagata H, Kaji N, Kamiya H, Baba Y, Harashima H, Biol. Pharm. Bull., 29, 2451 (2006)

[21] Murtaza I, Marra G, Schlapbach R, Patrignani A, Kunzli M, Wagner U, Sabates J, Dutt A, Biotechnol. Appl. Biochem., 45, 29 (2006)

[22] Allantaz F, Chaussabel D, Banchereau J, Pascual V, Curr. Opin. Immunol., 19, 623 (2007)

[23] Driouch K, Landemaine T, Sin S, Wang S, Lidereau R, Clin. Exp. Metastasis, 24, 575 (2007)

[24] Welsh JB, Zarrinkar PP, Sapinoso LM, Kern SG, Behling CA, Monk BJ, Lockhart DJ, Burger RA, Hampton GM, Proc. Natl. Acad. Sci. USA, 98, 1176 (2001)

[25] Golub TR, Slonim DK, Tamayo P, Huard C, Gaasenbeek M, Mesirov JP, Coller H, Loh ML, Downing JR, Caligiuri MA, Bloomfield CD, Lander ES, Science, 286, 531 (1999)

[26] Hu JS, Durst M, Kerb R, Truong V, Ma JT, Khurgin E, Balaban D, Gingeras TR, Hoffman BB, Ann. NY Acad. Sci., 919, 9 (2000)

[27] Zisman A, Peroni OD, Abel ED, Michael MD, Mauvais-Jarvis F, Lowell BB, Wojtaszewski JF, Hirshman MF, Virkamaki A, Goodyear LJ, Kahn CR, Kahn BB, Nat. Med., 6, 924 (2000)

[28] Wada J, Zhang H, Tsuchiyama Y, Hiragushi K, Hida K, Shikata K, Kanwar YS, Makino H, Kidney Int., 59, 1363 (2001)

[29] Dhahbi JM, Mote PL, Cao SX, Spindler SR, Diabetes Technol. Ther., 5, 411 (2003)

[30] Susztak K, Bottinger E, Novetsky A, Liang D, Zhu Y, Ciccone E, Wu D, Dunn S, McCue P, Sharma KK, Diabetes, 53, 784 (2004)

[31] Mi H, Lazareva-Ulitsky B, Loo R, Kejariwal A, Vandergriff J, Rabkin S, Guo N, Muruganujan A, Doremieux O, Campbell MJ, Kitano H, Thomas PD, Nucleic Acids Res., 33, D284 (2005)

[32] Ohtsuka Y, Agishi Y, Nippon Rinsho., 50, 1631 (1992)

[33] El-Demerdash FM, Yousef MI, El-Naga NI, Toxicol., 43, 57 (2005)

[34] Potter PM, Wolverton JS, Morton CL, Whipple DO, Danks MK, Cytometry, 32, 223 (1998)

[35] Zhu W, Song L, Zhang H, Matoney L, Lecluyse E, Yan B, Drug Metab. Dispos., 28, 186 (2000)

[36] Yamada T, Hosokawa M, Sotoh T, Moroo I, Takahashi M, Akatsu H, Brain Res., 658, 163 (1994)

[37] Satoh T, Hosokawa M, Annu. Rev. Pharmacol. Toxicol., 38, 257 (1998)

[38] Xu G, Zhang Q, Ma MK, McLeod HL, Clin. Cancer Res., 8, 2605 (2002)

[39] Dobrzyn A, Ntambi JM, Obes. Rev., 6, 169 (2005)

[40] Foster DW, Ann. NY Acad. Sci., 1033, 1 (2004)

[41] Nishida M, Sasaki T, Terada H, Kawada J, Experientia., 44, 756 (1988)

[42] Waters KM, Ntambi JM, J. Biol. Chem., 269, 27773 (1994)

[43] Ramesh B, Pugalendi KV, Yale J. Biol. Med., 78, 189 (2005)

[44] Ooi GT, Tseng LY, Rechler MM, Biochem. Biophys. Res. Commun., 189, 1031 (1992)

[45] Rodgers BD, Bautista RM, Nicoll CS, Proc. Soc. Exp. Biol. Med., 210, 234 (1995)