Increased production of S-adenosyl-L-methionine using recombinant Saccharomyces cerevisiae sake K6

Eun-Sil Choi; Bu-Soo Park; Sang-Woo Lee; Min-Kyu Oh

Korean Journal of Chemical Engineering, Vol.26, No.1, 156-159, January, 2009

DOI10.1007/s11814-009-0025-x Export Citation

Increased production of S-adenosyl-L-methionine using recombinant Saccharomyces cerevisiae sake K6

Eun-Sil Choi, Bu-Soo Park¹, Sang-Woo Lee, and Min-Kyu Oh^†

Department of Chemical & Bioengineering, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul 136-713, Korea
¹Samyang Genex, 63-2 Hwaam-dong, Yuseong-gu, Daejeon 305-717, Korea

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S. cerevisiae sake K6 was the firstly isolated industrial strain to overproduce S-adenosyl-L-methionine (SAM). Although the strain has advantages over other strains, such as GRAS (generally recognized as safe) property, the S. cerevisiae K6 has not been further developed with DNA recombinant technology due to the lack of a proper genetic marker. In this study, UV mutagenesis was conducted with S. cerevisiae sake K6. With the method, a mutant of sake yeast with leucine auxotroph, K6-1, was isolated. The mutant showed comparable growth rate and SAM productivity with its wild type. Using the auxotroph as a genetic marker, a SAM synthase in S. cerevisiae, SAM2, was overexpressed in the mutant strain. This recombinant DNA technology successfully increased SAM productivity in sake yeast.

Keywords:S-Adenosyl-L-methionine;Saccharomyces cerevisiae Sake;Auxotrophic Mutant;Recombinant DNA

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[2] Roje S, Phytochemistry, 67, 1686 (2006)

[3] Izu H, Shobayashi M, Manabe Y, Goto K, Iefuji H, Biosci. Biotechnol. Biochem., 70, 2982 (2006)

[4] Hosea Blewett HJ, Crit. Rev. Food Sci. Nutr., 48, 458 (2008)

[5] Shippy RA, Mendez D, Jones K, Cergnul I, Karpiak SE, BMC Psychiatry, 4, 38 (2004)

[6] Scarpa S, Fuso A, D’Anselmi F, Cavallaro RA, Febs Lett., 541, 145 (2003)

[7] Shiozaki S, Shimizu S, Yamada H, J. Biotechnol., 4, 345 (1986)

[8] Shiozaki S, Shimizu S, Yamada H, Agr. Biol. Chem. Tokyo, 53, 3269 (1989)

[9] Shiomi N, Fukuda H, Fukuda Y, Murata K, Kimura A, J. Ferment. Bioeng., 71, 211 (1991)

[10] Shobayashi M, Mukai N, Iwashita K, Hiraga Y, Iefuji H, Appl. Microbiol. Biotechnol., 69(6), 704 (2006)

[11] Li DY, Yu J, Tian L, Ji XS, Yuan ZY, Chin. J. Biotechnol., 18, 295 (2002)

[12] He JY, Deng JJ, Zheng YH, Gu J, J. Biotechnol., 126, 519 (2006)

[13] Chen HX, Chu J, Zhang SL, Zhuang YP, Qian JC, Wang YH, Hu XQ, Appl. Microbiol. Biotechnol., 74(6), 1205 (2007)

[14] Zhang JG, Wang XD, Zhang JN, Wei DZ, J. Biosci. Bioeng., 105, 335 (2008)

[15] Thomas D, Rothstein R, Rosenberg N, Surdin-Kerjan Y, Mol. Cell. Biol., 8, 5132 (1988)

[16] Chan SY, Appling DR, J. Biol. Chem., 278, 43051 (2003)