Effects of pH, salt type, and ionic strength on the second virial coefficients of aqueous bovine serum albumin solutions

YoonKook Park; Ginkyu Choi

Korean Journal of Chemical Engineering, Vol.26, No.1, 193-198, January, 2009

DOI10.1007/s11814-009-0032-y Export Citation

Effects of pH, salt type, and ionic strength on the second virial coefficients of aqueous bovine serum albumin solutions

YoonKook Park^†, and Ginkyu Choi¹

Department of Chemical System Engineering, Hongik University, Jochiwon-up, Yongi-gun 339-701, Korea
¹Departmnet of Electronic and Electrical Engineering, Hongik University, Jochiwon-up, Yongi-gun 339-701, Korea

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The osmotic pressures of aqueous bovine serum albumin (BSA) solutions were measured at different pH (3.6, 4.6, 5.6, and 7.6) in combination with different concentrations (0.01M, 0.1M, 1M, and 3M) of salts (sodium, pottasium, and lithum) by using a Wescor colloid membrane osmometer. The osmotic second virial coefficients for BSA were determined from the experimental osmotic pressure. Predominant forces between protein molecules were measured at the various pH, ionic strength, and type of salt. These experimental data were utilized to determine the depth of square-well potential, which accounts for specific interactions between protein molecules at various conditions.

Keywords:Second Virial Coefficients;Specific Interaction;SBA;Osmotic Pressure;PMF

[References]

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[2] Ramirez-Alvarado M, Merkel JS, Regan L, PNAS, 97, 8979 (2000)

[3] Uversky VN, Karnoup AS, Khurana R, Segel DJ, Doniach S, Fink AL, Protein Sci., 8, 161 (1999)

[4] Tessier PM, Lenhoff AM, Sandler SI, Biophys. J., 82, 1620 (2002)

[5] Oakley AJ, Wilce MC, Clin. and Exp. Pharm. Physio., 27, 145 (2000)

[6] Sun Z, Cai J, Korean J. Chem. Eng., 23(4), 607 (2006)

[7] Kuehner DE, Heyer C, Ramsch C, Fornefeld UM, Blanch HW, Prausnitz JM, Biophys. J., 73, 3211 (1997)

[8] Hitscherich Jr. C, Kaplan J, Allaman M, Wiencek J, Loll PJ, Protein Sci., 9, 1559 (2000)

[9] Ducruix A, Guilloteau JP, Ries-Kautt M, Tardieu A, Journal of Crystal Growth, 168, 28 (1996)

[10] Velev OD, Kaler EW, Lenhoff AM, Biophys. J., 75, 2682 (1998)

[11] Wu JZ, Prausnitz JM, Fluid Phase Equilib., 155(1), 139 (1999)

[12] Starling EH, J. Physiol., 24, 317 (1899)

[13] Moon YU, Curtis RA, Anderson CO, Blanch HW, Prausnitz JM, Journal of Solution Chem., 29, 699 (2000)

[14] McMillan Jr. WG, Mayer JE, J. Chem. Phys., 13, 276 (1945)

[15] Vilker VL, Colton CK, Smith KA, J. Colloid Interface Sci., 79, 548 (1981)

[16] Verwey EJW, Overbeek JTG, Theory of stability of lyophobic colloids, Elsevier, Amsterdam (1948)

[17] Nir S, Prog. Surf. Sci., 8, 1 (1977)

[18] Wertheim MS, J. Chem. Phys., 85, 2929 (1986)

[19] Tanford C, Swanson SA, Shore WS, J. Am. Chem. Soc., 77, 6414 (1955)

[20] Pujar NS, Zydney AL, J. Colloid Interface Sci., 192(2), 338 (1997)

[21] Tanford C, Adv. Protein Chemistry, 23, 121 (1968)

[22] Curtis RA, Prausnitz JM, Blanch HW, Biotechnol. Bioeng., 57(1), 11 (1998)

[23] Hofmeister F, Arch. Exp. Pathol. Pharmakol., 24, 247 (1888)

[24] Arakawa T, Timasheff SN, Biochem., 21, 6545 (1982)

[25] Chang BH, Bae YC, Biomacromolecules, 4(6), 1713 (2003)

[26] Collins KD, Biophys. J., 72, 65 (1997)

[27] Korolev N, Lyubartsev AP, Rupprecht A, Nordenskiold L, Biophys. J., 77, 2736 (1999)

[28] Vogel R, Fan GB, Sheves M, Siebert F, Biochem., 40, 483 (2001)

[29] von Hippel P, Schleich T, Acc. Chem. Res., 2, 257 (1969)

[30] Muta H, Kojima R, Kawauchi S, Tachibana A, Satoh M, J. Mol. Structure: THEOCHEM, 536, 219 (2001)

[31] Moon YU, Anderson CO, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 168(2), 229 (2000)

[32] Alberty RA, Marvin Jr. HH, J. Am. Chem. Soc., 73, 3220 (1951)

[33] Kuehner DE, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 116(1-2), 140 (1996)

[34] Zhang FJ, Skoda MWA, Jacobs RMJ, Martin RA, Martin CM, Schreiber F, J. Phys. Chem. B, 111(1), 251 (2007)

[35] Curtis RA, Ulrich J, Montaser A, Prausnitz JM, Blanch HW, Biotechnol. Bioeng., 79(4), 367 (2002)

[36] Rescic J, Vlachy V, Jamnik A, Glatter O, J. Colloid Interface Sci., 239(1), 49 (2001)

[37] Haynes CA, Tamura K, Korfer HR, Blanch HW, Prausnitz JM, J. Phys. Chem., 96, 905 (1992)

[38] Yousef MA, Datta R, Rodgers VGJ, J. Colloid Interface Sci., 243(2), 321 (2001)