The purpose of this study was to prepare and characterize inclusion complex of 7-dehydrocholesterol(7-DHC) with hydroxypropyl-β-cyclodextrin(HPβCD). The inclusion complex seen to good solubility contrast with physical mixture. The inclusion efficiency detennined by HPLC analysis was 83.72 %. Additionally, the inclusion complex was characterized by UV- VIS spectrophotometry and fourier transfonn infrared specσoscopy(FT-IR). This results demonstrated that 7-dehydrocholesterol with hydroxypropyl-β-cyclodextrin fonned an inclusion complex.