Immuno-strip biosensor system to detect enrofloxacin residues

Young-Kee Kim; Hajin Kim

Journal of Industrial and Engineering Chemistry, Vol.15, No.2, 229-232, March, 2009

DOI10.1016/j.jiec.2008.10.007 Export Citation

Immuno-strip biosensor system to detect enrofloxacin residues

Young-Kee Kim^{1, 2, †}, and Hajin Kim¹

¹Department of Chemical Engineering and Program of Integrated Biotechnology, Hankyong National University, Anseong, Kyonggi-do 456-749, Republic of Korea
²Institute of Chemical Technology, Hankyong National University, Anseong, Kyonggi-do 456-749, Republic of Korea

E-mail:

The immuno-strip biosensor was developed by combination of immuno-chromatography assay and sandwich enzyme linked immunosorbent assay (ELISA) techniques to detect enrofloxacin, a widely used broad-spectrum antibiotic. A proposed analytical system, consisted of immuno-reaction step and enzyme reaction step, has several advantages such as a very cheap manufacturing cost, a short analysis time (approximately 20 min), an easy-to-use, a high sensitivity, and a qualitatively confirmation by bare eyes. Enzyme reaction step in a proposed analytical tool could enhance the signal of immunosensor, resulting in the high sensitivity. A proposed system was successfully applied to detect enrofloxacin in a range of 100.10000 ppb. The lower detection limit was 100 ppb, which is themaximum residue limit of enrofloxacin in animal product.

Keywords:Immunosensor;ELISA;Immuno-chromatography assay;Enrofloxacin;Colorimetric

[References]

Marchesini GR, Haasnoot W, Delahaut P, Gercek H, Nielen MWF, Anal. Chim. Acta, 586, 259 (2007)
Garlucci G, J. Chromatogr. A, 812, 343 (1998)
Wolfson JS, Hooper DC, Am. J. Med., 91, 153 (1991)
Bimazybute MA, Rozet E, Dizier I, Gustin P, Hubert P, Crommen J, Chiap P, J. Chromatogr. A, 1189, 456 (2008)
Hermo MP, Barron D, Barbosa J, Anal. Chim. Acta, 539, 77 (2005)
EEC, Off. J. Eur. Communities, L125, 10 (1996)
EEC Commission Regulation, No. 2377/90, modified in Reg. 508/99, L60/16 of 9/3/99, Brussels (1999)
Holtzapple K, Buckley A, Stanker H, J. Chromatogr. B, 754, 1 (2001)
Lee SY, Lee SJ, Jung HT, J. Ind. Eng. Chem., 9(1), 9 (2003)
Ramos M, Aranda A, Garcia E, Reuvers T, Hooghuis H, J. Chromatogr. B, 789, 373 (2003)
Schneider MJ, Darwish AM, Freeman DW, Anal. Chim. Acta, 586, 269 (2007)
Schneider MJ, Donoghue DJ, J. Chromatogr. B, 780, 83 (2002)
Granelli K, Branzell C, Anal. Chim. Acta, 586, 289 (2007)
Bucknall S, Silverlight J, Coldham N, Thorne L, Jackman R, Food Addit. Contam., 20, 221 (2003)
Giles JS, Hariharan H, Heaney SB, New Microbiol., 19, 155 (1994)
Haasnoot W, Gercek H, Cazenier G, Nielen MW, Anal. Chim. Acta, 586, 312 (2007)
Huet AC, Charlier C, Tittlemier SA, Singh G, Benrejeb S, Delahaut P, J. Agric. Food Chem., 54, 2822 (2006)
Cao L, Lin H, Mirsky VM, Anal. Chim. Acta, 589, 1 (2007)
Choi JW, Kim YK, Kim HJ, Lee W, Seong GH, J. Microbiol. Biotechnol., 16, 1229 (2006)
Hwangbo HJ, Yun BH, Cha JS, Eur. J. Pharm. Sci., 18, 197 (2003)
Wang ZH, Zhu Y, Ding SY, He FY, Beier RC, Li JC, Jiang HY, Feng CW, Wan YP, Zhang SX, Kai ZP, Yang XL, Shen JZ, Anal. Chem., 79, 4471 (2007)
Zhang S, Liu Z, Zhou N, Wang Z, Shen J, Anal. Chim. Acta, 612, 83 (2008)
Kim YK, Biochip J., 1, 145 (2007)
Paek SH, Cho JH, Cho IH, Kim YK, Oh BK, Biochip J., 1, 1 (2007)
Thermo Fisher Scientific Inc., Instructions for EZ-Link Activated Antibody Labeling Kit, Doc. No. 0266, Rockford, IL, USA

[Referenced By]

[1] Marchesini GR, Haasnoot W, Delahaut P, Gercek H, Nielen MWF, Anal. Chim. Acta, 586, 259 (2007)

[2] Garlucci G, J. Chromatogr. A, 812, 343 (1998)

[3] Wolfson JS, Hooper DC, Am. J. Med., 91, 153 (1991)

[4] Bimazybute MA, Rozet E, Dizier I, Gustin P, Hubert P, Crommen J, Chiap P, J. Chromatogr. A, 1189, 456 (2008)

[5] Hermo MP, Barron D, Barbosa J, Anal. Chim. Acta, 539, 77 (2005)

[6] EEC, Off. J. Eur. Communities, L125, 10 (1996)

[7] EEC Commission Regulation, No. 2377/90, modified in Reg. 508/99, L60/16 of 9/3/99, Brussels (1999)

[8] Holtzapple K, Buckley A, Stanker H, J. Chromatogr. B, 754, 1 (2001)

[9] Lee SY, Lee SJ, Jung HT, J. Ind. Eng. Chem., 9(1), 9 (2003)

[10] Ramos M, Aranda A, Garcia E, Reuvers T, Hooghuis H, J. Chromatogr. B, 789, 373 (2003)

[11] Schneider MJ, Darwish AM, Freeman DW, Anal. Chim. Acta, 586, 269 (2007)

[12] Schneider MJ, Donoghue DJ, J. Chromatogr. B, 780, 83 (2002)

[13] Granelli K, Branzell C, Anal. Chim. Acta, 586, 289 (2007)

[14] Bucknall S, Silverlight J, Coldham N, Thorne L, Jackman R, Food Addit. Contam., 20, 221 (2003)

[15] Giles JS, Hariharan H, Heaney SB, New Microbiol., 19, 155 (1994)

[16] Haasnoot W, Gercek H, Cazenier G, Nielen MW, Anal. Chim. Acta, 586, 312 (2007)

[17] Huet AC, Charlier C, Tittlemier SA, Singh G, Benrejeb S, Delahaut P, J. Agric. Food Chem., 54, 2822 (2006)

[18] Cao L, Lin H, Mirsky VM, Anal. Chim. Acta, 589, 1 (2007)

[19] Choi JW, Kim YK, Kim HJ, Lee W, Seong GH, J. Microbiol. Biotechnol., 16, 1229 (2006)

[20] Hwangbo HJ, Yun BH, Cha JS, Eur. J. Pharm. Sci., 18, 197 (2003)

[21] Wang ZH, Zhu Y, Ding SY, He FY, Beier RC, Li JC, Jiang HY, Feng CW, Wan YP, Zhang SX, Kai ZP, Yang XL, Shen JZ, Anal. Chem., 79, 4471 (2007)

[22] Zhang S, Liu Z, Zhou N, Wang Z, Shen J, Anal. Chim. Acta, 612, 83 (2008)

[23] Kim YK, Biochip J., 1, 145 (2007)

[24] Paek SH, Cho JH, Cho IH, Kim YK, Oh BK, Biochip J., 1, 1 (2007)

[25] Thermo Fisher Scientific Inc., Instructions for EZ-Link Activated Antibody Labeling Kit, Doc. No. 0266, Rockford, IL, USA