화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.17, No.6, 424-429, June, 2009
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Biological Synthesis of Alkyne-terminated Telechelic Recombinant Protein
Niraikulam Ayyadurai, So-Yeon Kim1, Sun-Gu Lee1, †, Soundrarajan Nagasundarapandian2, Aleya Hasneen2, Hyun-Jong Paik2, Seong Soo A. An3, and Eugene Oh4
  • School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea
  • 1Institute for Environmental Technology and Industry, Pusan National University, Busan 609-735, Korea
  • 2Department of Polymer Science and Chemical Engineering, Pusan National University, Busan 609-735, Korea
  • 3Gachon BioNano Research Institute, Kyungwon University, Gyeonggi-do 461-701, Korea
  • 4Graduate School of Global Entrepreneurship, Hoseo University, Chungnam 336-795, Korea
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In this study, we demonstrate that the biological unnatural amino acid incorporation method can be utilized in vivo to synthesize an alkyne-terminated telechelic protein. Synthesis of terminally-functionalized polymers such as telechelic polymers is recognized to be important, since they can be employed usefully in many areas of biology and material science, such as drug delivery, colloidal dispersion, surface modification, and formation of polymer network. The introduction of alkyne groups into polymeric material is particularly interesting since the alkyne group can be a linker to combine other materials using click chemistry. To synthesize the telechelic recombinant protein, we attempted to incorporate the L-homopropargylglycine into the recombinant GroES fragment by expressing the recombinant gene encoding Met at the codons for both N- and C-terminals of the protein in the Met auxotrophic E. coli via Hpg supplementation. The Hpg incorporation rate was investigated and the incorporation was confirmed by MALDI-TOF analysis of the telechelic recombinant protein.
Keywords:recombinant protein;telechelic protein;unnatural amino acid;alkynylation.
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