Beneficial Effects of Microwave-Induced Argon Plasma Treatment on Cellular Behaviors of Articular Chondrocytes Onto Nanofibrous Silk Fibroin Mesh

Soo Chang Jin; Hyun Sook Baek; Yeon I Woo; Mi Hee Lee; Jung-Sung Kim; Jong-Chul Park; Young Hwan Park; Dong Kyun Rah; Kie-Hyung Chung; Seung Jin Lee; In Ho Han

Macromolecular Research, Vol.17, No.9, 703-708, September, 2009

Soo Chang Jin^{1, 2}, Hyun Sook Baek¹, Yeon I Woo^{1, 2}, Mi Hee Lee^{1, 2}, Jung-Sung Kim^{1, 2}, Jong-Chul Park^{1, 2, †}, Young Hwan Park³, Dong Kyun Rah⁴, Kie-Hyung Chung⁵, Seung Jin Lee⁶, and In Ho Han⁷

¹Department of Medical Engineering, Yonsei University College of Medicine, Seoul 120-752, Korea
²Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea
³Department of Biosystems and Biomaterials Science and Engineering, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Korea
⁴Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine, Seoul 120-752, Korea
⁵Physico-Technology Laboratory, Korea Accelerator and Plasma Research Association, Gangwon-do 269-843, Korea
⁶College of Pharmacy, Ewha Womans University, Seoul 120-750, Korea
⁷Institute for Clinical Dental Research, Korea University, Seoul 136-701, Korea

E-mail:

Silk fibroin scaffolds were examined as a biomaterial option for tissue-engineered cartilage-like tissue. In tissue engineering for cartilage repair using a scaffold, initial chondrocyte-material interactions are important for the following cell behaviors. In this study, the surface of nanofibrous silk fibroin (NSF) meshes was modified by a microwave-induced argon plasma treatment in order to improve the cytocompatibility of the meshes used as cartilaginous grafts. In addition, the effects of a plasma treatment on the cellular behavior of chondrocytes on NSF were examined. The plasma treatment resulted in an increase in the hydrophilicity of NSF meshes suggesting that the cytocompatibility of the mesh might be improved. Furthermore, the human articular chondrocytes showed higher viability on the surface-modified NSF meshes. These results suggest that the surface modification of NSF meshes by plasma can enhance the cellular behavior of chondrocytes and may be used in tissue engineering.

Keywords:nanofibrous silk fibroin;chondrocytes;cartilage;microwave-induced argon plasma;cytocompatibility

[References]

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[Referenced By]

[1] Ozcan C, Hasirci N, J. Biomater. Sci. Polym. Ed., 18, 759 (2007)

[2] Pesskova V, Kubies D, Hulejova H, Himmlova L, J. Mater. Sci. Mat. Med., 18, 465 (2007)

[3] Keselowsky G, Collard DM, Garcia AJ, Proc. Natl. Acad. Sci. U.S.A., 102, 5953 (2005)

[4] Williams SF, Martin DP, Horowitz MD, Peoples OP, Int. J. Biol. Macromol., 25, 111 (1999)

[5] Sacristan J, Reinecke H, Mijangos C, Polymer, 41(15), 5577 (2000)

[6] Sheu MS, Hoffman AS, Feijen J, J. Adhesion Sci. Technol., 6, 995 (1992)

[7] Chan-Park MB, Zhang J, Gao JX, Liu EJ, J. Adhesion Sci. Technol., 16, 1883 (2002)

[8] Sun X, Liu J, Lee ML, J. Appl. Polym. Sci. Anal. Chem., 80, 856 (2008)

[9] Karageorgiou V, Meinel L, Hofmann S, Malhotra A, Volloch V, Kaplan D, J. Biomed. Mater. Res. A, 71, 528 (2004)

[10] Lee KH, Baek DH, Ki CS, Park YH, Int. J. Biol. Macromol., 41, 168 (2007)

[11] Park BJ, Lee DH, Park JC, Lee IS, Lee KY, Hyun SO, Chun MS, Chung KH, Phys. Plasmas, 10, 4539 (2003)

[12] Lee KY, Park BJ, Lee DH, Lee IS, Hyun SO, Chung KH, Park JC, Surf. Coat. Technol., 193, 35 (2005)

[13] Jeong L, Yeo IS, Kim HN, Yoon YI, Jang DH, Jung SY, Min BM, Park WH, Int. J. Biol. Macromol., 44, 222 (2009)

[14] Chen JY, Wan GJ, Leng YX, Yang P, Sun H, Wang J, Huang N, Surf. Coat. Technol., 186, 270 (2004)

[15] Yokota T, Terai T, Kobayashi T, Meguro T, Iwaki M, Surf. Coat. Technol., 201, 8048 (2007)

[16] Jin HJ, Kaplan DL, Nature, 424, 1057 (2003)

[17] Aoki H, Tomita N, Morita Y, Hattori K, Harada Y, Sonobe M, Wakitani S, Tamada Y, Biomed. Mater. Eng., 13, 309 (2003)

[18] Hofmann S, Knecht S, Stussi E, Langer R, Kaplan D, Vunjak-Novakovic G, Merkle HP, Meinel L, Tissue Eng., 12, 2729 (2006)

[19] Wang Y, Blasioli DJ, Kim HJ, Kim HS, Kaplan DL, Biomaterials, 27, 4434 (2006)

[20] Lin L, Zhou C, Wei X, Hou Y, Zhao L, Fu X, Zhang J, Yu C, Arthritis Rheum, 58, 1067 (2008)