Biotechnology Letters, Vol.30, No.3, 513-519, 2008
The insecticidal crystal protein Cry2Ab10 from Bacillus thuringiensis: cloning, expression, and structure simulation
The cry2Ab-type gene was cloned from Bacillus thuringiensis and designated as cry2Ab10. The recombinant Cry2Ab10 protein expressed in E. coli cells shows high toxicity against Plutella xylostella. The protein structure was constructed by homology modeling, and the receptor-binding sites were predicted by a molecular docking method.
Keywords:Bacillus thuringiensis;Cry2Ab10;diamondback moth;homology modeling;insecticidal activity;molecular docking;protein structure and modelling