The mixing effect of induced-charge electrokinetic flow (ICEKF) in a rectangular microchannel with embedded conducting hurdles is investigated in this paper. A correction method is suggested to estimate numerically the induced zeta potential on the conducting surface. Two-dimensional pressure-linked Navier-Stokes equation is used to model the flow field in the channel. The numerical results show flow circulations generated from the induced non-uniform zeta potential distribution along the conducting hurdle surfaces. It is demonstrated that the local flow circulations provide effective means to enhance the flow mixing between different solutions. The mixing enhancement effect is experimentally validated using PDMS based microchannels with embedded platinum hurdles. The dependence of the degree of mixing enhancement on the hurdle geometries and hurdle numbers is also predicted. The mixing using ICEKF described in this paper can be used in various microfluidics and lab-on-a-chip applications. (C) 2008 Elsevier Ltd. All rights reserved.