The kinetics of anodic oxidation of sodium gluconate was studied. The reciprocal of apparent current shows the reciprocal dependence on the concentration of sodium gluconate. The kinetic model correlated well with the experimental results. The rate determining step was found to be the anodic oxidation of gluconate anion to form gluconate free radical. The current of the anodic oxidation of sodium gluconate can be expressed as i = 0.89[NaC6H11O7]/(0.7357 + [NaC6 H11O7])*exp(0.37F eta(2)/RT) where F is Faraday's constant and eta(2), is the overpotantial of forming gluconate free radical from gluconate anion.