The dynamic transesterification reaction of peanut oil in supercritical methanol media was investigated. The reaction temperature and pressure were in the range of 250 degrees C-310 degrees C and 10.0 MPa-16.0 Mpa, respectively. The molar ratio of peanut oil to methanol was 1:30. It was found that the yield of methyl esters was higher than 90% under the supercritical methanol. The apparent reaction order and activation energy of transesterification was 1.5 and 7.472 kJ/mol, respectively. In this method, the reaction time was shorter and the processing was simpler than that of the common acid catalysis transesterification.