참당귀(Angelica gigas Nakai) 뿌리 추출물의 화장품소재 특성

박숙경; 홍슬기; 김희진; 김보영; 김타곤; 강재선; 김동욱; Suk Kyoung Park; Seul-Ki Hong; Hee Jin Kim; Bo Young Kim; Tagon Kim; Jae Seon Kang; Donguk Kim

Korean Chemical Engineering Research, Vol.47, No.5, 553-557, October, 2009

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참당귀(Angelica gigas Nakai) 뿌리 추출물의 화장품소재 특성

Cosmetic Effect of Angelica gigas Nakai Root Extracts

박숙경, 홍슬기¹, 김희진², 김보영³, 김타곤³, 강재선⁴, 김동욱 ^{3, †}

코리아나화장품, 331-833 충남 천안시 성거읍 점촌리 204-1
¹바이오메틱스, 621-749 경남 김해시 어방동 607번지 성산관 310호
²시노펙스, 790-841 경북 포항시 남구 대송면 송동리 856번지
³인제대학교 제약공학과, 621-749 경남 김해시 어방동 607번지
⁴경성대 약학대학, 608-736 부산시 남구 대연동 314-79

Suk Kyoung Park, Seul-Ki Hong¹, Hee Jin Kim², Bo Young Kim³, Tagon Kim³, Jae Seon Kang⁴, and Donguk Kim^{3, †}

Coreana Cosmetics, 204-1 Jeongchon-ri Seonggeo-eup Cheonan-si, Chungnam 331-833, Korea
¹Biometics, Inje University, 310 Sunsang-guan, Gimhae, Gyeongnam 621-749, Korea
²SYNOPEX, 856 Songdong-ri, Daesong-myen, Nam-gu, Pohang, Gyeongbuk 790-841, Korea
³Department of Pharmaceutical Engineering, Inje University, 607, Obang-dong, Gimhae, Gyongnam 621-749, Korea
⁴Department of Pharmacy, Kyungsung University, 314-79 Daeyeon-dong, Nam-gu, Busan 608-736, Korea

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초록

참당귀 뿌리 추출물에서 기능성 화장품소재를 개발하고자 하였다. 기기분석시험을 통하여 참당귀 뿌리 추출물에는 유효성분으로서 decursin과 decursinol angelate가 약 97%로 매우 고농도로 존재하였으며 그 비가 약 3:2로 나타났다. 화장품소재 시험으로는 항산화(DPPH free radical scavenging assay), 미백(Tyrosinase inhibition assay, Melanogenesis inhibition assay), 주름개선(Elastase inhibition assay), 자외선 흡수 및 안전성 시험(MTT assay)이 실시되었다. 참당귀 뿌리 추출물은 15 μg/ml의 농도에서 tyrosine에 대한 tyrosinase 저해효과가 45.2±3.9%, melanin 생성억제 효과가 24.2±12.0%로 미백효과가 우수하였다. 항산화효과는 240 μg/ml의 추출물농도에서 DPPH free radical 소거율이 40.9±9.1%로 비교적 우수하였다. 주름개선 효과는 100 μg/ml의 추출물농도에서 12.7±6.8%로 낮았으며, 자외선 흡수효과도 거의 없었다. 따라서 본 연구를 통하여 참당귀 뿌리 추출물은 화장품용 미백소재로서 가능성을 보여주었다.

Root extracts of Angelica gigas Nakai were tested to see the possibility for functional cosmetic agents. From ethanol extraction method, 97% of decursin and decursinol angelate was obtained, and concentration ratio of decursin to decursinol angelate was about 3:2. To test possibility as a functional cosmetic agent, DPPH free radical scavenging assay, UVA/B absorption, tyrosinase inhibition assay, melanogenesis inhibition assay, elastase inhibition assay and MTT assay were done. Root extracts of Angelica gigas Nakai showed 45.2±3.9% tyrosinase inhibition of tyrosine, and 24.2±12.0% melanin inhibition at 15 μg/ml extract concentration, so that it indicated good whitening effect. DPPH free radical scavenging activity was 40.9±9.1% at 240 μg/ml concentration, which is relatively good. Anti-wrinkle effect was poor such that it was 12.7±6.8% at 100 μg/ml. UVA/B absorption was also negligible. From the research, root extracts of Angelica gigas Nakai showed good potential as a whitening agent.

Keywords:Angelica Gigas Nakai;Decursin;Antioxidation;Whitening;Anti-Wrinkle

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[1] http://www.khidi.or.kr/index.do.

[2] Korea Food and Drug Administration, http://www.kfda.go.kr/index.html.

[3] Elsner P, Mailbach HI, Cosmeceuticals and Active Cosmetics, 2nd ed., Taylor & Francis, New York, NY (2005)

[4] Vielhaber G, Schmaus G, Jacobs K, Franke H, Lange S, Hermann M, Joppe H, Koch O, IFSCC, 9, 227 (2006)

[5] Sohn E, Development Trend of Natural Cosmetic Agents, KISTI Tech Trend Report (2003)

[6] Kim HS, Lee CW, Kim DH, Kim GO, Kim SJ, Chang IS, J Soc Cosmet Scientists Korea, 33, 69 (2007)

[7] Kim JY, Yang HJ, Lee KH, Jeon SM, Ahn YJ, Won BR, Park SN, J Soc Cosmet Scientists Korea, 33, 165 (2007)

[8] Rudolph T, Buhle P, Beck J, Plucker F, Reiffen KA, Buchlolz H, IFSCC, 9, 227 (2006)

[9] Pharmacognosy Researchers, Modernpharmacognosy, Hak Chang Publishing, Seoul (2000)

[10] Kang JS, Korea Patent No. 10-0893779-0000 (2009)

[11] Blois MS, Nature, 181, 1199 (1958)

[12] Ishihara Y, Oka M, Tsunakawa M, Tomita K, Hatori M, Yamamoto H, Kamei H, Miyaki T, Konishi M, Oki T, J. Antibiotics, 44, 25 (1991)

[13] Kim JH, Sim GS, Lee DH, Lee GS, Lee BC, Pyo HB, J Soc Cosmet Scientists Korea, 33, 203 (2007)

[14] Yang HJ, Ahn YJ, Kim JH, Park SN, J Soc Cosmet Scientists Korea, 34, 189 (2008)