In this study, an efficient procedure was developed for callus induction and regeneration of kiwifruit (Actinidia deliciosa) using different organs of shoots developed under in vitro conditions. Effects of explants source and media (M-1, 1.0 mg l(-1) BA + 2.0 mg l(-1) 2,4-D-M-2, 1.0 mg l(-1) NAA + 2.0 mg l(-1) 2,4-D) on initiation of callus were examined in order to obtain callus for organogenesis. The best callus for plant regeneration was obtained from leaf explants on Murashige and Skoog's medium (MS) supplemented with M-2. Formation of callus from leaf of kiwifruit (A. deliciosa) was cultured in MS medium containing different concentration of N-6-benzylaminopurin (BA; 0.0, 1.0, 2.0, 4.0, 6.0, 8.0 mg l(-1)) for callus proliferation and plant regeneration. Although the first shoot formation was appeared in medium containing 6.0 and 8.0 mg l(-1) BA, the best shoots formation was obtained in medium with 4.0 mg l(-1) BA.