The potential use of biomass of Aeromonas hydrophila for biosorption of chromium from aqueous solution was investigated. The variables (PH, initial Cr(VI) concentration, biomass dose, and temperature) affecting process were optimized by performing minimum number of experimental runs with the help of central composite design. The results predicted by design were found to be in good agreement (R-2=99.1%) with those obtained by performing experiments. Multiple regression analysis shows that uptake decreases with increase in PH and biomass dose, whereas it increases with increase in temperature and concentration. The maximum removal of Cr(VI) predicted by contour and optimization plots was 184.943 mg/g at PH 1.5, initial Cr(VI) concentration 311.97 mg/L, temperature 60 degrees C, and biomass dose 1.0 g. The removal of Cr(VI) was governed by adsorption of Cr(VI) as well as its reduction into Cr(III), which further gets adsorbed. The sorption capacity of biomass was calculated from experimental data using Langmuir sorption model and was found to be 151.50 mg/g at 40 degrees C and PH 1.5, which is comparable to other biosorbents. In addition to this, Dubihin-Radushkevich model was applied, and it was found that nature of sorption was chemisorption.