Applied Biochemistry and Biotechnology, Vol.159, No.1, 24-32, 2009
Endoxanthanase, a Novel beta-d-Glucanase Hydrolyzing Backbone Linkage of Intact Xanthan from Newly Isolated Microbacterium sp XT11
A novel endoxanthanase was produced and isolated from the culture of Microbacterium sp. XT11 growing on xanthan. Optimum pH and temperature for the enzyme activity were 6.0 and 35-40 A degrees C, respectively. The endoxanthanase cleaves the backbone endo-beta-1,4-glucosidic linkage of the xanthan molecule, which is specific to the intact xanthan. However, the lyase-treated xanthan or carboxymethyl cellulose could not be cleaved by endoxanthanase.