In order to obtain dense and stable poly( ethylene oxide) ( PEO) layers for reducing protein adsorption, polystyrene ( PS) plates were first soaked in chloroform/methanol mixed organic solvent to swell the polymer. The swelled PS plates were then immersed into Pluronic F127 ( amphiphilic block copolymer) aqueous solution, Pluronic F127 molecules were adsorbed favorably on the swelled PS surfaces. After evaporation of organic solvent, the adsorbed Pluronic F127 molecules were trapped and anchored permanently on the PS substrates. The dense and stable PEO layers anchored on the PS surfaces can effectively inhibit protein adsorption. (c) 2008 Elsevier B. V. All rights reserved.