The C:H and C:N:H films were prepared by direct current magnetic filtered cathodic vacuum arc deposition (DC-MFCVA) at room temperature (RT). Raman spectra were used to analyze structure of films. The surface energy and interfacial tension of the films were calculated by contact angle measure using four common liquids. Platelet adhesion experiment in vitro was done to characterize anticoagulant property of the synthesized films from an aspect. The results showed that the doping of N and H element could decrease interfacial tension of DLC films. The synthesized DLC have good anticoagulant property and may be considered as candidate anticoagulant biomaterials. (C) 2008 Published by Elsevier B.V.