High energy femtosecond laser process was employed to create regular surface patterning on titanium while sand blasting treatment made a coarse surface. Both laser-induced titanium and blasted titanium could promote the formation of calcium phosphate compounds after the acid and alkali treatment, but little crystallized hydroxyapatite was grown on the laser-induced titanium in 1.5SBF only for 6 h, whereas Ca4P6O19 was formed on the sand-blasted titanium. The femtosecond laser process together with common acid and alkali treatment might provide potential choice to enhance the biocompatibility of titanium and its alloys. (C) 2008 Elsevier B.V. All rights reserved.