Ti6Al4V alloy is one of the most widely used materials for biomedical implants. Among its properties, it is remarkable the photoactivity displayed by its passive layer, which is mainly composed by titanium dioxide. However, variations in the processing conditions may yield to differences in the microstructure which can be reflected on the surface properties of the machined product. From contact angle measurements taken on different zones of samples removed from a commercial bar of Ti6Al4V, it has been shown that the modi. cations of the surface Gibbs energy suffered by the alloy under UV irradiation have a radial dependence. This behaviour is related to slight microstructural changes of the alloy, particularly with an increase in the volume fraction of the beta-phase when moving to the interior of the sample, which alters the composition and/or microstructure of the passive layer along its radius. This study shows that gradients in the microstructure and physical properties are sample size dependent and are likely related to thermal gradients during processing. (C) 2009 Elsevier B.V. All rights reserved.