This work examines the inhibitory effect of TCEP on the in vitro fibrillation of lien lysozyme at pH 2. We demonstrate that the inhibition of lien lysozyme fibrillation by TCEP follows a close-dependent manner. Our data show that the addition of TCEP, prevents alpha-to-beta transition and promoted unfolding of lysozyme. Moreover, Our findings suggested that the TCEP-induced attenuated fibrillation is associated with disulfide disruption and structural unfolding of HEWL. (C) 2009 Elsevier Inc. All rights reserved.