Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

Yong-Qian Fu; Qing Xu; Shuang Li; Yao Chen; He Huang

Korean Journal of Chemical Engineering, Vol.27, No.1, 183-186, January, 2010

DOI10.1007/s11814-009-0323-3 Export Citation

Strain improvement of Rhizopus oryzae for over-production of fumaric acid by reducing ethanol synthesis pathway

Yong-Qian Fu, Qing Xu, Shuang Li, Yao Chen, and He Huang^†

College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-OrientedChemical Engineering, Nanjing University of Technology, Nanjing 210009, P. R. China

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Rhizopus oryzae mutants were isolated from the wild type strain ME-F01 after mutagenesis with UV coupled with nitrosoguanidine (NTG) and the following allyl alcohol resistance selection method. By analyzing the activities of alcohol dehydrogenase (ADH) involved in ethanol synthesis pathway and batch fermentation, one mutant, ME-UN-8, was isolated that produced 21.1% more fumaric acid than ME-F01 with the corresponding byproduct of ethanol decreased by 83.7%.

Keywords:Alcohol Dehydrogenase;Allyl Alcohol;Fumaric Acid;Mutation;Rhizopus oryzae

[References]

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[2] Anon, Ind. Biopro., 27, 11 (2005)

[3] Ling LB, Ng TK, US Patent 4,877,731 (1989)

[4] Riscaldati E, Moresi M, Federici F, Petruccioli M, Biotechnol. Lett., 22(13), 1043 (2000)

[5] Kenealy W, Zaady E, Dupreez JC, Stieglitz B, Goldberg I, Appl. Environ. Microbiol., 52, 128 (1986)

[6] Osmani SA, Scrutton MC, Eur. J. Biochem., 47, 119 (1985)

[7] Peleg Y, Battat E, Scrutton MC, Goldberg I, Appl. Microbiol. Biotechnol., 32, 334 (1989)

[8] Cao NJ, Du JX, Gong CS, Tsao GT, Appl. Environ. Microbiol., 62, 2926 (1996)

[9] Zhou Y, Du J, Tsao GT, Bioproc. Biosys. Eng., 25, 179 (2002)

[10] Du JX, Cao NJ, Gong CS, Tsao GT, Yuan NJ, Appl. Biochem. Biotech., 63, 541 (1997)

[11] Arpornwichanop A, Shomchoam N, Korean J. Chem. Eng., 24(1), 11 (2007)

[12] Skory CD, Freer SN, Bothast RJ, Biotechnol. Lett., 20(2), 191 (1998)

[13] Zheng Z, Luo SZ, Li XJ, Wu XF, Pan LJ, Jiang ST, Afr. J. Biotechnol., 8, 280 (2009)

[14] Suntornsuk W, Hang YD, Lett. Appl. Microbiol., 19, 249 (1994)

[15] Tsao GT, Cao NJ, Du J, Gong CS, Adv. Biochem. Eng. Biot., 65, 243 (1999)

[16] Osmani SA, Scrutton MC, Eur. J. Biochem., 133, 551 (1983)

[17] Rhodes RA, Moyer AJ, Smith ML, Kelley SE, Appl. Microbiol., 7, 74 (1959)

[18] Gangl IC, Weigand WA, Keller FA, Appl. Biochem. Biotech., 28, 471 (1991)

[19] Bai DM, Zhao XM, Li XG, Xu SM, Biochem. Eng. J., 18, 41 (2004)