Thermo-responsive porous membranes with grafted linear and crosslinked poly(N-isopropylacrylamide) (PNIPAM) gates are successfufly prepared at temperatures above and below the lower critical solution temperature (LCST) of PNIPAM by using a plasma-induced grafting polymerization method, and the effects of operation pressure and grafting temperature on the thermo-responsive gating characteristics of the prepared membranes are investigated systematically. The fluxes of water through the grafted membranes increase simply with increasing the operation pressure no matter whether the environmental temperature is 40 degrees C or 25 degrees C. Under high operation pressure (e.g., higher than 0.14 MPa), the grafted linear PNIPAM gates deform to a certain extent, whereas the grafted crosslinked PNIPAM gates do not deform. For both membranes with grafted linear and crosslinked PNIPA-M gates, the membranes prepared at 25 degrees C (below the LCST of PNIPAM) show larger thermo-responsive gating coefficients than those prepared at 40 degrees C (above the LCST of PNIPAM), which results from different distributions of grafted PNIPAM gates in the membrane pores. When the PNIPAM gates are grafted at 25 degrees C, the grafted layer near the membrane surface is much thicker than that inside the membrane pores; on the other hand, when the PNIPAM gates are grafted at 40 degrees C, the grafted layer is homogeneously formed throughout the whole pore length. Both linear and crosslinked grafted PNIPAM gates in the membrane pores exhibit stable and repeatable thermo-responsive "open-close" switch performances under the operation pressure of 0.26 MPa. The results in this study provide valuable guidance for designing, fabricating, and operating thermo-responsive gating membranes with desirable performances.