Lactic acid has long been widely used in many applications. Currently, the worldwide market is increasing due to the discovery of biodegradable polylactic acid. In this work, L-lactic acid separations from filamentous fungal fermentation broth using ion-exchange chromatography and in-house electrodialysis, were studied and compared. Dowex Marathon WBA was used for the lactic acid separation. The adsorption equilibrium followed a Langmuir isotherm. The optimal conditions for lactic acid adsorption in a fixed-bed column were at pH 6.0, and 0.8 mL/min and elution by a mixture of 1.0 M sulfuric acid and 1.0 M phosphoric acid in a ratio of 30:70 at 0.3 mL/min. The final lactic acid recovery was 76 % with 90 % purity. A laboratory scale in-house electrodialysis apparatus was constructed with an effective membrane area of 2.925 . 10(-3) m(2). The effects of feeding solution concentration, flow rate, pH of the fermentation broth, and applicable voltage were studied. Under the optimal conditions, lactic acid recovery was 92 % with 100 % purity and a specific energy consumption of 0.6122 kWh/kg.