The present work describes a novel method for the isolation of the neutral N-containing fraction from crude oil using column liquid chromatography on silica gel chemically modified with Ti(IV) oxide using a grafting method. The crude oil sample is introduced onto the column and eluted with a proper sequence of eluents. The first eluted fraction contains only bydrocarbons. The N-containing compounds are eluted in a isolated fraction. Both fractions are collected, concentrated and analyzed using HRGC with NPD detection. The method is shown to be very selective for the isolation of the neutral N-containing fractions from crude oil.