The cellular localization of a potent fat-accumulation inhibitor (-)-ternatin was investigated. FITC-labeled ternatin, a chemical probe for current study, was synthesized from a highly bioactive ternatin analogue using Click Chemistry, which was found to be bioactive. The treatment of 3T3-L1 murine preadipocytes with synthetic probe revealed that (-)-ternatin is localized in a specific organelle of 3T3-L1 cells.