Effect of Nonlamellar-Prone Lipids on Protein Encapsulation in Liposomes

Taeho Ahn; Youn-Tae Chi; Chul-Ho Yun

Macromolecular Research, Vol.17, No.12, 956-962, December, 2009

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Effect of Nonlamellar-Prone Lipids on Protein Encapsulation in Liposomes

Taeho Ahn^†, Youn-Tae Chi¹, and Chul-Ho Yun^{1, †}

Department of Biochemistry, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea
¹School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea

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We investigated the effect of two nonlamellar-prone lipids, phosphatidylethanolamine (PE) and dioleoylglycerol (DOG), on the efficiency of protein encapsulation in liposomes. When the phosphatidylcholine (PC) matrix was replaced with PE or DOG during liposome formulation, the amounts of glutathione S-transferase and bovine serum albumin entrapped in the vesicles increased with increasing PE or DOG concentration. The presence of PE and DOG synergistically affected protein entrapment. These results suggest that protein encapsulation can be enhanced by the presence of nonlamellar lipids and/or lipid-induced membrane properties.

Keywords:protein encapsulation;nonlamellar-prone lipids;liposomes

[References]

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Epand RM, Bottega R, Biochem. Biophys. Acta, 944, 144 (1988)
Epand RM, Biochemistry, 24, 7092 (1985)
Das S, Rand RP, Biochemistry, 25, 2882 (1986)
Schwendener RA, Adv. Exp. Med. Biol., 620, 117 (2007)
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[1] Medina OP, Zhu Y, Kairemo K, Curr. Pharm. Des., 10, 2981 (2004)

[2] Winterhalter M, Lasic DD, Chem. Phys. Lipids, 64, 35 (1993)

[3] Jr. Szoka F, Papahadjopoulos D, Annu. Rev. Biophys. Bioeng., 9, 467 (1980)

[4] Jr. Szoka F, Papahadjopoulos D, Proc. Natl. Acad. Sci. USA, 75, 4194 (1978)

[5] Lichtenberg D, Barenholtz Y, Methods Biochem. Anal., 33, 337 (1988)

[6] Kirby C, Gregoriadis G, J. Microencapsul., 1, 33 (1984)

[7] Ahn T, Guengerich FP, Yun CH, Biochemistry, 37, 12860 (1998)

[8] Kolchens S, Ramaswami V, Birgenheier J, Nett L, O’Brien DF, Chem. Phys. Lipids, 65, 1 (1993)

[9] Vaskovsky VE, Kostetsky EY, Vasendin IM, J. Chromatogr., 114, 1291 (1975)

[10] Stern I, Shapiro B, J. Clin. Pathol. (London), 6, 158 (1953)

[11] Habig WH, Jakoby WB, Methods Enzymol., 77, 398 (1981)

[12] Ahn T, Yun CH, Arch. Biochem. Biophys., 369, 288 (1999)

[13] Lundback AK, Haneskog L, Andersson L, Heijbel A, Ingemarsson L, Birse D, Life Science News, 15, 10 (2003)

[14] Colletier JP, Chaize B, Winterhalter M, Fournier D, BMC Biotechnol., 2, 1 (2002)

[15] Ellens H, Bentz J, Szoka FC, Biochemistry, 25, 285 (1986)

[16] Perly B, Smith IC, Jarrel HC, Biochemistry, 24, 1055 (1985)

[17] Lewis RN, Mannock DA, McElhaney RN, Turner DC, Gruner SM, Biochemistry, 28, 541 (1989)

[18] Gordesky SE, Marinetti GV, Love R, J. Membr. Biol., 20, 111 (1975)

[19] Epand RM, Biochemistry, 24, 7092 (1985)

[20] Das S, Rand RP, Biochemistry, 25, 2882 (1986)

[21] Hincha DK, Oliver AE, Crowe JH, Biochim. Biophys. Acta, 1368, 150 (1998)

[22] Epand RM, Bottega R, Biochem. Biophys. Acta, 944, 144 (1988)

[23] Epand RM, Biochemistry, 24, 7092 (1985)

[24] Das S, Rand RP, Biochemistry, 25, 2882 (1986)

[25] Schwendener RA, Adv. Exp. Med. Biol., 620, 117 (2007)

[26] Huang SL, Adv. Drug Deliv. Rev., 60, 1167 (2008)

[27] Skeie S, Int. Dairy J., 4, 573 (1994)

[28] Hwang TL, Lee WR, Hua SC, Fang JY, J. Dermatol. Sci., 46, 11 (2007)