Fibrin microbeads (FMB) are made by moderate-heat condensation of fibrin drops in oil to obtain beads in the range of 100-200 mu m. This procedure yields protein-based hard stable beads with high binding capability of the matrix dependent cell. Therefore, the FMB could serve for high yield isolation of mesenchymal stein cells (MSC) from different sources in higher yield than that obtained by conventional methods. The cells could be expanded on the FMB in suspension culture without the need for trypsinization and passages. Cell differentiation into different phenotypes, such as bone and cartilage, can be induced in cells loaded on FMB. When implanted, the cells on FMB Survive the implantation and can download to become integrated with the target organs for tissue regeneration. This makes the use of FMB a simple technique for cell-based tissue regeneration without the need for implanting bulk scaffolds on which the cells have a low rate of survival. FMB technology provides a simple and highly effective fibrin-based method for cell separation, expansion in suspension and delivery to assist tissue regeneration.