In this paper the aqueous electrochemiluminescence (ECL) from polymethylmethacryl ate Langmuir-Blodgett films incorporating water insoluble luminophors as rubrene (RUB) and 9,10-diphenylanthracene (DPA) at the electrode was obtained. ECL was excited using reaction with coreactant tripropylamine in aqueous solution in the anodic potential range using scanning voltammetry method. Dependence of ECL response of RUB films versus their thickness and coreactant concentration was also studied. (c) 2008 Elsevier Ltd. All rights reserved.