Electrophoresis, Vol.29, No.24, 4944-4947, 2008
On-chip tryptic digest with direct coupling to ESI-MS using magnetic particles
As a step toward a fully automated front-end microfluidic chip for MS proteomics, we propose a system capable of performing online tryptic digest and ESI-MS, using a replaceable on-chip digestion microcolumn based on self-assembled magnetic particles.