This paper deals with the problem for waste water reduction from the culture medium step of an antibiotic production in a case of a heat integrated fermentation stage. The heat integration of the fermentation stage introduces a heat integration cycle time that decreases the time between consecutive inoculation phases of the fermenters and arises as a precondition for redistribution of the seed fermenters used in a way the waste water from the process to be decreased. To explore this potential for waste reduction, a scheduling procedure for the culture medium step is introduced involving the following main steps: determination of a time horizon for the seed preparation step; creation of schedules with a zero-release of waste water, called basic schedules and, relaxation of the basic schedules to ensure a success of inoculation. Finally, different schedules are created for the culture medium step, according to proposed procedure, and investigated from the waste reduction and the required pieces of equipment points of views.