Cattle brain is rich in lipids including cholesterol. As a alternative process to extract cholesterol, the ecofriendly solvent supercritical carbon dioxide (SC CO2), along with different cosolvents such as acetone, 2-propanol, and ethanol, was used in an attempt to understand the effect of cosolvents. A maximum of 53% of the total cholesterol in the brain sample could only be extracted either using pure SC CO2 or using cosolvents along with SC CO2. Although the total amount of cholesterol extracted did not differ with the addition of cosolvents, the rate of extraction was found to increase significantly with the addition of cosolvents in small quantities. The kinetics of extraction using pure SC CO2 as well SC CO2 with cosolvents was modeled using conventional differential mass balance equation for packed beds. The effective diffusivity coefficient was found to double with cosolvent acetone, while found to be more than three times with cosolvents 2-propanol and ethanol in comparison with the pure SC CO2. The effective diffusivity coefficient was found to be of the order of 10(-13) m(2)/s, for extraction of cholesterol from cattle brain, either with SC CO2 or SC CO2 with cosolvents.