Binuclear copper complex (2-[bis(2-aminoethyl)amino]ethanol, 4,4'-bipyridine bridged dicopper(II) complex) was grafted onto the surface of a glassy carbon electrode (GCE) using the cyclic voltammetric method in a phosphate buffer solution (PBS). The modified electrode resulted in efficient electrocatalytic activity for anodic oxidation of uric acid (UA) and ascorbic acid (AA) via a substantial decrease in anodic over-potentials for both compounds. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using this modified electrode result in two well-resolved anodic waves for the oxidation of UA and AA in mixed solution, making possible the simultaneous determination of both compounds. Linear analytical curves were obtained in the ranges 5.0-300.0 mu M and 5.0-160.0 mu M for UA and AA concentrations through DPV methods, respectively. The detection limits were 2.0 mu M of UA and AA. This electrode was used for UA and AA determinations in urine samples with satisfactory results.