Journal of Applied Microbiology, Vol.107, No.4, 1298-1307, 2009
In vitro efficacy of nisin Z against Candida albicans adhesion and transition following contact with normal human gingival cells
Aim: To investigate the nisin Z innocuity using normal human gingival fibroblast and epithelial cell cultures, and its synergistic effect with these gingival cells against Candida albicans adhesion and transition from blastospore to hyphal form. Methods and Results: Cells were cultured to 80% confluence and infected with C. albicans in the absence or presence of various concentrations of nisin Z. Our results indicate that only high concentrations of nisin Z promoted gingival cell detachment and differentiation. Determination of the LD50 showed that the fibroblasts were able to tolerate up to 80 mu g ml