One of the major themes in tissue engineering is scaffold fabrication. The porosity and pore size of scaffolds play a critical role in tissue engineering. Different methods are used to measure the porosity and pore size of scaffolds, although none can predict the cell infiltration for various cell sizes, shapes, and configurations. The aim of this study was to predict the cell infiltration of various cells with different sizes, shapes, and configurations through the use of image analysis. In this study, cell models were used to predict cell infiltration into nanofiber scaffolds. The results of this study showed that with increases in the cell size and the number of layers of nanofibers, the number of cells that could infiltrate the scaffolds decreased. In addition, the cell configuration had some effect on cell infiltration into the nanofiber scaffolds. (C) 2008 Wiley Periodicals, Inc. J Appl Polym Sci 111: 317-322, 2009