Norfloxacin-molecular imprinted polymer was prepared by bulk polymerization with Norfloxacin (NFXC) as template molecule, Methacrylic acid (MAA) as functional monomer and Trimethylolpropane Trimethacrylate (TRIM) as cross linking agent. And the imprinted polymer membranes of polysulfone-matrix were also prepared by blending method. The structures of Norfloxacin-molecular imprinted polymer were measured and confirmed by spectra of FTIR and TEM, respectively. Although the combination characteristic and mechanism of this molecular recognition membrane were studied by scan electro-microscope (SEM) and combinative equation experiment, the results showed that the molecular recognition membrane represented high selectivity for Norfloxacin. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 113: 1125-1132,2009