Isolation and characterization of a novel antistress gene from Chlamydomonas sp W80

Suda Y; Yoshikawa T; Okuda Y; Tsunemoto M; Tanaka S; Ikeda K; Miyasaka H; Watanabe M; Sasaki K; Harada K; Bamba T; Hirata K

Journal of Bioscience and Bioengineering, Vol.107, No.4, 352-354, 2009

DOI10.1016/j.jbiosc.2008.12.019 Export Citation

Isolation and characterization of a novel antistress gene from Chlamydomonas sp W80

Suda Y, Yoshikawa T, Okuda Y, Tsunemoto M, Tanaka S, Ikeda K, Miyasaka H, Watanabe M, Sasaki K, Harada K, Bamba T, Hirata K

Here we report the isolation and characterization of the Chlamydomonas sp. W80-derived Go ATP synthase subunit II WCFII homologue gene. Intracellular ATP concentration and stress-tolerance of Escherichia coli were increased by transformation with WCFII. We propose that WCFII-mediated stress tolerance is due to enhanced accumulation of intracellular ATP. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Chlamydomonas;Anti-stress gene;Green alga;ATP synthase;subunit CFo-II