A laboratory sequencing batch biofiltration reactor (SBBR) packed with 1 inch zeolite-containing polyethylene media was used to remove organic matter and nutrients from synthetic wastewater. The effects of the anoxic/aerobic time ratio and the mass ratio of chemical oxygen demand to total nitrogen (known as C/N mass ratio) on the performance of SBBR were investigated. Throughout the experiment, the anoxic/aerobic time and C/N mass ratios varied ranging from 0.1-1.0, and 3-10, respectively. The SBBR had a chemical oxygen demand (COD) removal efficiency of 98% as well as a total nitrogen (T-N) removal efficiency of up to 97%, and a total phosphorous (T-P) removal efficiency of 67%. These highest efficiencies were obtained at the anoxic/aerobic time ratio of 0.5 and the C/N mass ratio of 10.