Formation of polymeric spheres in nanometer to sub-micrometer is achieved through a spin-coating process with polyamide (from 4,4'-(hexafluoroisopropylidene)dianiline and 4,4'-dicarboxydiphenylether) solutions in N,N-dimethylformamide and N,N-dimethylacetamide. The formation of polymeric spheres and their sizes are dependent on the polymer solution concentrations and the rates in spin-coating. The polymeric spheres could be obtained on various substrates including silicon, mica, and glass. (C) 2008 Elsevier Inc. All rights reserved.