Spectral and physiochemical behavior of water/sodium bis-(2-ethylhexyl)sulfosuccinate (AOT)/isooctane (ME-I) and water/AOT + lecithin (LC)/isooctane (ME-II) microemulsions has been investigated in the presence of organodiselenides. It was found that the OH, CO and C-O-C FTIR stretching frequency of the formulated microemulsions change with the addition of organodiselenides. Interaction with Acridine Orange (AO) indicates organodiselenide presence at the interface. Estimates of the micropolarities of the microemulsions at different water contents were carried out in terms of an empirical polarity parameter E-T(30). The values increase with the increase in omega = [H2O]/[surfactant]. The emission spectra of AO reveal the quenching capacities of the added organodiselenides. The temperature induced percolation process has also been studied. The results indicate a Subsequent increase in solubilization of organodiselenide in ME-II as compared to ME-I. (C) 2009 Elsevier Inc. All rights reserved.