Stable lyophilisomes of fibrinogen at pH 7.5 have been prepared by the method Of a rapid freezing-heating and annealing sequence. Reduction of the lyophilisomes of the nickel-fibrinogen complex coated on solid substrates and Subsequent heating showed formation of nickel hydroxide and finally nickel oxide. Ultraviolet-visible spectroscopy has been used to monitor the thin films Of pure fibrinogen microcapsules, as well as the Subsequent nucleation and growth of nanoparticles within the supramolecular structure. Transmission electron microscopy showed initially a thread-like Structure which disappeared oil continued heating, resulting in nanoparticles ranging from 10 to 50 nm. Particle-size distribution Of product was analyzed by using X-ray diffraction (XRD), transmission electron microscopy (TEM) and the corresponding selected area electron diffraction (SAED), and Brunauer-Emmett-Teller (BET) N-2 adsorption. The results Suggest that the NiO particles have a body-centered cubic Structure and are well dispersed. The particle-size distribution ranges from 10 to 50 nm with an average particle size about 28 rim, and the specific surface area is 34 m(2)/g. Magnetic study carried out on the prepared nanoparticles showed a ferromagnetic behavior. (C) 2009 Elsevier Inc. All rights reserved.