Biosorption of Cr(VI) and Ni(H) by a fused yeast from Candida tropicalis and Candida lipolytica Under varying range of pH. initial metal concentration and reaction time was investigated. Net cation release and Cr removal reached 2.000 mmol/l and 81.37% when treating 20 mg/l Cr(VI) at pH 2 with 25 mg/l biomass for 30 min, while for Ni were 0.351 mmol/l and 64.60%. respectively. Trace metal elements such as Co, Col. Mn, Mo, Se and Zn played active role in biosorption as important ingredients of functional enzymes. Cr(VI) was reduced to less toxic Cr(III) and chelated with extracellular secretions, and further accumulated inside the cells. For Ni biosorption, however. largely a passive uptake process influenced by ion gradient led to lower adsorption capacity and cations release. Fourier transform infrared (FTIR) spectrum analysis indicated that amide and pyridine on cells were involved in binding With Cr, bill for Ni. bound-OH and nitro-compounds were the main related functional groups. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) analysis confirmed that considerable amounts of metals precipitated on cell surface when dealing with high concentration metals. (C) 2008 Elsevier B.V. All rights reserved.