This study presents a method to regenerate molecularly imprinted polymers (MIPs) used for the selective removal of endocrine disrupting Compounds from aqueous effluents. Regeneration was based on solvent extraction under UV irradiation to regenerate the polymer and the solvent while destroying the contaminants. Acetone was selected as the best solvent for irradiation of estrone (E1), 17 beta-estradiol (E2) and ethinylestradiol (EE2) using either UVC (254 nm) or UV-vis. A MIP synthesized with E2 as template was then tested for the extraction of this compound from a 2 mu g/L loaded aqueous solution. E2 was recovered by 73 +/- 11% and 46 +/- 13% from the MIPs and a non-imprinted control polymer synthesized under the same conditions, respectively, after a single step elution with acetone. The irradiated polymers and acetone were reused for an additional extraction regeneration cycle and showed no capacity decrease. (C) 2008 Elsevier B.V. All rights reserved.