The effect of Tween80, Mn(II) and veratryl alcohol (VA) on the production of ligninolytic enzymes of Phanerochaete chrysosporium in a packed-bed bioreactor using small pieces of Kissiris as carrier, was investigated. The results of the enzyme activities were noticeable in terms of decolorization and COD removal of the textile effluent containing an azo dye (Astrazon Red FBL). No dilution was made on the tested textile effluent and it was not sterilized, also. Maximum decolorization of the dye (87%) and COD removal (42%), both occurred when only Tween80 (0.05%, w/v) was added to the effluent. The maximum activities of lignin peroxidase (LiP) and manganese peroxidase (MnP) were (U/I): 17 and 52. respectively. The role of MnP was pronounced in the dye decolorization process, while the influence of UP was noticeable on COD removal. The reusability of the original biomass was examined by replacing undiluted textile effluent (i e., five times). The cellular performance of the original biomass in repeated-batch operations was promising. (C) 2009 Elsevier B.V. All rights reserved.