It was shown previously that the self-supported films of nanocrystalline (30-80 nm) BaTiO3 spontaneously split into regions, within which the direction of spontaneous polarization of each grain is aligned as closely as possible to some average direction. These regions, called polycrystalline macro-domains, may be of two types: (1) those with out-of-plane polarization and (2) those with in-plane polarization. In-plane macro-domains exhibit in-plane optical anisotropy which can be monitored using cross-polarized transmitted light. This property was utilized in the current work to study the influence of temperature variation on macro-domains. According to the temperature dependence of the intensity of cross-polarized transmitted light, the films could be divided into three groups: (group 1) those films that did not exhibit strong changes in intensity; (group 2) those that exhibited a strong and abrupt change at a temperature between 20 and 120 A degrees C; and (group 3) those that upon heating exhibited a gradual increase in the intensity of the cross-polarized transmitted light. The observed changes were reversible and consistent with a 90A degrees rotation of the c-axis of some grains, which caused reversible changes in the macro-domain structure.