Two negatively charged polyelectrolyte complex colloidal nanoparticles (PEC-) and one positively charged nanoparticle (PEC+) were prepared and used as novel layer-by-layer (LbL) building blocks. These PEC nanoparticles include poly(2-methacryloyloxy ethyl trimethylammonium chloride)/sodium carboxymethyl cellulose (PDMC/CMCNa PEC-), poly(diallyldimethylammonium chloride)/CMCNa (PDDA/CMCNa PEC-) and PDDA/poly(sodium-p-styrenesulfonate) (PDDA/PSS PEC+). LbL multilayer films based on (PEC+/PEC-) were constructed on both quartz slides and modified polyamide (MPA) reverse osmosis support membranes. UV-vis spectroscopy, quartz crystal microbalance (QCM), field emission scanning microscopy (FESEM) and atomic force microscopy (AFM) were utilized to follow the thickness growth and morphology evolution of these multilayer films with increasing bi-layer numbers. LbL multilayer films deposited on MPA support membranes were subjected to pervaporation dehydration of 10 wt% water-isopropanol and effect of bi-layer numbers and feed temperature on pervaporation performance was studied. Generally, PEC+/PEC- can be LbL self-assembled successfully on both substrates with a thickness growth rate ca. 200 nm/bi-layer. Moreover, PEC+/PEC- multilayer films show high pervaporation performance with film thickness up to several micrometers. For example, performance of the multilayer films in dehydrating 10 wt% water-isopropanol at 50 degrees C is J = 1.18 kg/m(2) h, alpha = 1013 for (PEC+/PDMC-CMCNa PEC-)(24) and J = 1.36 kg/m(2) h, alpha = 938 for (PEC+/PDMC-CMCNa PEC-)(25), respectively. (C) 2009 Elsevier B.V. All rights reserved.