Recovery of cellulases by adsorption/desorption using cation exchange resins

Jin Chuan Wu; Kiat Rui Ng; Joanne Chong; Kai Jie Yang; Xiao Ping Lam; Chai Teck Nam; Aninto Jati Nugroho

Korean Journal of Chemical Engineering, Vol.27, No.2, 469-473, February, 2010

DOI10.1007/s11814-010-0096-8 Export Citation

Recovery of cellulases by adsorption/desorption using cation exchange resins

Jin Chuan Wu^†, Kiat Rui Ng, Joanne Chong, Kai Jie Yang, Xiao Ping Lam, Chai Teck Nam, and Aninto Jati Nugroho

Institute of Chemical & Engineering Sciences, Agency for Science, Technology and Research (A*Star), 1 Pesek Road, Jurong Island, Singapore 627833

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Cellulases from Trichoderma reesei were recovered by adsorption in sodium acetate buffer at lower pH using cation exchange resins followed by desorption at higher pH. The weakly acidic ion exchange resin WK10 was found to be the best among the six resins tested in terms of the enzyme activity recovery. The optimal pH values for the adsorption and desorption were 4.0 and 8.0, respectively, and the optimal adsorption and desorption times were both 5 h. Almost 100% of the initial cellulase activity was recovered under the optimal conditions with the supplement of β-glucosidase, which was unable to be efficiently recovered due to its strong adsorption (95.7%) but poor desorption (1.9%).

Keywords:Cellulase;β-Glucosidase;Adsorption;Desorption;Ion Exchange Resin;Activity Recovery

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[Referenced By]

[1] Lin Y, Tanaka S, Appl. Microbiol. Biotechnol., 69(6), 627 (2006)

[2] McMillan JD, Renew. Energy, 10, 295 (1997)

[3] Saha BC, Iten LB, Cotta MA, Wu YV, Process Biochem., 40, 3693 (2005)

[4] Um BH, Karim MN, Henk LL, Appl. Biochem. Biotechnol., 105-108, 115 (2003)

[5] Chung YC, Bakalinsky A, Penner MH, Appl. Biochem. Biotechnol., 121-124, 947 (2003)

[6] Nakamura Y, Sawada T, Inoue E, J. Chem. Technol. Biotechnol., 76(8), 879 (2001)

[7] Palmarola-Adrados B, Calbe M, Zacchi G, Appl. Biochem. Biotechnol., 113-116, 989 (2004)

[8] Kristensen JB, Borjesson J, Bruun MH, Tjerneld F, Jorgensen H, Enzyme Microb. Technol., 40(4), 888 (2007)

[9] Kim SB, Chun JW, Appl. Biochem. Biotechnol., 113-116, 1023 (2003)

[10] Pan X, Xie D, Gilkes N, Gregg DJ, Saddler JN, Appl. Biochem. Biotechnol., 121-124, 1069 (2005)

[11] Lee J, J. Biotechnol., 56, 1 (1997)

[12] Szczodrak J, Fiedurek J, Biomass Bioenerg., 10(5-6), 367 (1996)

[13] Wen Z, Liao W, Chen S, Appl. Biochem. Biotechnol., 121-124, 93 (2005)

[14] Adsul MG, Varma AJ, Gokhale DV, Green Chem., 9, 58 (2007)

[15] Ramos LP, Breuil C, Saddler JN, Enzyme Microb. Technol., 15, 19 (1993)

[16] Steele B, Rai S, Nghiem J, Stowers M, Appl. Biochem. Biotechnol., 121-124, 901 (2005)

[17] Evans CS, Appl. Microbiol. Biotechnol., 22, 128 (1985)

[18] Negro MJ, Manzanares P, Ballesteros I, Oliva JM, Cabanas A, Ballesteros M, Appl. Biochem. Biotechnol., 105-108, 87 (2003)

[19] Hayashida S, Mo K, Hosoda A, Appl. Environ. Microbiol., 54, 1523 (1988)

[20] OH KD, KIM C, Korean J. Chem. Eng., 4(2), 105 (1987)

[21] Singh J, Batra N, Sobti RC, J. Ind. Microbiol. Biotechnol., 31, 51 (2004)

[22] Hu CY, Lin LP, World J. Microbiol. Biotechnol., 19, 515 (2003)

[23] Li YH, Ding M, Wang J, Xu GJ, Zhao FK, Appl. Microbiol. Biotechnol., 70(4), 430 (2006)