Transfer rate measurement of lysozyme by liquid-liquid extraction using reverse micelles with dense CO2

Sun-Mi Jung; Un-Mi Shin; Md. Salim Uddin; Sun-Young Park; Hideki Kishimura; Gordon Wilkinson; Byung-Soo Chun

Korean Journal of Chemical Engineering, Vol.27, No.2, 596-601, February, 2010

DOI10.1007/s11814-010-0063-4 Export Citation

Transfer rate measurement of lysozyme by liquid-liquid extraction using reverse micelles with dense CO2

Sun-Mi Jung, Un-Mi Shin, Md. Salim Uddin, Sun-Young Park¹, Hideki Kishimura², Gordon Wilkinson³, and Byung-Soo Chun^†

Institute of Food Science, Faculty of Food Science & Biotechnology, Pukyong National University, Busan 608-737, Korea
¹School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, Australia
²Research Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan
³School of Chemical Engineering, University of Adelaide, Adelaide, SA 5005, Australia

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Lysozyme was extracted from aqueous solution into i-octane using reverse micelles in the presence of pressurized CO2. A squat vessel with two independent stirrers was used to measure the mass transfer of the lysozyme across a planar interface. Mass transfer coefficient, k(L) of the lysozyme from the aqueous to the organic phase was measured at selected ionic strengths, pH, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) surfactant concentrations, temperatures and pressurized CO2. The mass transfer rate of lysozyme was higher in high temperature (318 K) and pressure (20MPa). pH of 9 in aqueous phase showed highest mass transfer rate of lysozyme. The application of pressurized CO2 markedly increased the mass transfer rate of lysozyme comparing to conventional non-pressurized system.

Keywords:Lysozyme;Reverse Micelle;Mass Transfer;Pressurized CO2;Sodium Bis(2-ethylhexyl) Sulfosuccinate (AOT)

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[2] Lu Q, Chen H, Li K, Shi Y, Biochem. Eng. J., 1, 45 (1998)

[3] Nagayama K, Matsuura S, Doi T, Imai M, J. Mol. Cat. B, 4, 25 (1998)

[4] Lye GJ, Asenjo JA, Pyle DL, AIChE J., 42(3), 713 (1996)

[5] Nishiki T, Sato I, Muto A, Kataoka T, Biochem. Eng. J., 1, 91 (1998)

[6] Dekker M, Van’t Riet K, Bijsterbosch BH, Fijneman P, Hilhorst R, Chem. Eng. Sci., 45, 2949 (1990)

[7] Dungan SR, Bausch T, Hatton TA, Plucinski P, Nitsch W, J. Colloid Interface Sci., 145, 33 (1991)

[8] Lye GJ, Asenjo JA, Pyle DL, Chem. Eng. Sci., 49(19), 3195 (1994)

[9] Jarudilokkul S, Paulsen E, Stuckey DC, Bioseparation, 9, 81 (2000)

[10] Zhang HF, Lu J, Han BX, J. Supercrit. Fluids, 20(1), 65 (2001)

[11] Housaindokht MR, Haghighi B, Bozorgmehr MR, Korean J. Chem. Eng., 24(1), 102 (2007)

[12] Lewis JB, Chem. Eng. Sci., 3, 248 (1954)

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[15] Nishii Y, Nii S, Takahashi K, Takeuchi H, J. Chem. Eng. Jpn., 32(2), 211 (1999)

[16] Chun BS, Wilkinson GT, Sep. Sci. Technol., 37(14), 3323 (2002)

[17] Yoon HH, Korean J. Biotechnol. Bioeng., 5, 411 (1990)

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[19] Lye GJ, Asenjo JA, Pyle DL, in Separation Biotechnology, Volume 3, Pyle DL, Ed., Royal Society of Chemistry, UK (1994)

[20] Kim H, Baek K, Kim BK, Shin HJ, Yang JW, Korean J. Chem. Eng., 25(2), 253 (2008)

[21] Chun BS, Wilkinson GT, Ind. Eng. Chem. Res., 34(12), 4371 (1995)

[22] Asai S, Hatanaka J, Uekawa Y, J. Chem. Eng. Jpn., 16, 463 (1963)

[23] Park SW, Chun BS, Lim GT, Korean Chem. Eng. Res., 28, 594 (1990)

[24] Schneider GM, in Supercritical Fluids Fundamentals for Application, Kiran E, Sengers JMHL, Eds., Kluwer Academic Publishers, Boston, USA (1994)