Two donor-acceptor molecules with different pi-electron conjugative units, 1-((10-methyl-10H-phenothiazin-3-yl)ethynyl)anthracene-9,10-dione (AqMp) and 1,1'-(10-methyl-10H-phenothiazine-3.7-diyl)bis(ethyne-2,1-diyl)dianthrac ene-9,10-dione (Aq(2)Mp), have been synthesized and investigated for their photochemical and electrochemical properties. Density functional theory (DFT) calculations provide insights into their molecular geometry, electronic structures, and properties. These studies satisfactorily explain the electrochemistry of the two compounds and indicate that larger conjugative effect leads to smaller HOMO-LUMO gap (E-g) in Aq(2)MP, Both compounds show ICT and pi -> pi* transitions in the UV-visible range in Solution, and Aq(2)MP has a bathochromic shift and shows higher oscillator strength of the absorption, which has been verified by time-dependent DFT (TDDFT) calculations. The differences between AqMp and Aq(2)MP indicate that the structural and conjugative effects have great influence on the electronic properties of the Molecules.